Carbohydrate-Carbohydrate Binding of Ganglioside to Integrin α5 Modulates α5β1Function
نویسندگان
چکیده
منابع مشابه
Mena binds α5 integrin directly and modulates α5β1 function
Mena is an Ena/VASP family actin regulator with roles in cell migration, chemotaxis, cell-cell adhesion, tumor cell invasion, and metastasis. Although enriched in focal adhesions, Mena has no established function within these structures. We find that Mena forms an adhesion-regulated complex with α5β1 integrin, a fibronectin receptor involved in cell adhesion, motility, fibronectin fibrillogenes...
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Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins. The neoglycoconjugates comprise two active groups, saccharide and allyl, located on a poly(2-hydroxyethylacrylamide) backbone. The allyl group cross-links with the polyacrylamide gel matrix, while the saccharide groups are available for specific ...
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The extraordinarily high toxicity of botulinum neurotoxins primarily results from their specific binding and uptake into neurons. At motor neurons, the seven BoNT (botulinum neurotoxin) serotypes A-G inhibit acetylcholine release leading to flaccid paralysis. Uptake of BoNT/A, B, E, F and G requires a dual interaction with gangliosides and the synaptic vesicle proteins synaptotagmin or SV2 (syn...
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Lectins are cell-binding and agglutinating (glyco)proteins found in a variety of living forms. They will be defined here as homogeneous carbohydrate-binding (glyco)proteins principally of plant (seed) origin but also occurring in invertebrate (and vertebrate?) forms. In recent years, lectins have been employed in studying a wide range of biological phenomena including cell differentiation and m...
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The binding of carbohydrates to the hemelytic lectin CEL-III isolated from the marine inyertebrate dacumaria echinata was studied. Equilibrium dialysis data s"ggest that CEL-III has two carbohydrate-binding sites with equal aMnity. The binding of specific carbohydrates to CEL-III i"duces a decrease in the fluorescence intensity at 339 nm and the shift of the fluorescence emission maximum to a w...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2001
ISSN: 0021-9258
DOI: 10.1074/jbc.m006097200